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all cytokines except for human il-2  (PeproTech)


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    PeproTech all cytokines except for human il-2
    (a) Flow cytometric analysis of congenically marked OT-I CD8 T cells mixed ∼1:100 with bulk CD8 T cells and expanded with SIINFEKL-pulsed bone-marrow derived dendritic cells in IL-2 or cocktail of small molecules and <t>cytokines</t> (see methods). (b) Representative plots of expression of the phenotypic markers CD62L and CD44 on Thy1.2+ OT-I CD8 T cells. Plots in (a,b) representative of more than five independent experiments. (c) Plots of pre- and post-sort Thy1.2+ OT-I CD8 T cells grown as in (a) and purified by FACS for RNAseq analysis. (d) Unsupervised hierarchical clustering of RNAseq of populations sorted as in (c) showing all genes with a >2 fold difference between groups 1 and 3. Inset: principal component analysis of genes with >2-fold difference between any two groups for populations depicted in (c), and heatmap of selected biologically relevant genes in populations depicted in (c) and at selected time points of the OT-I response to L. monocytogenes Ova (from 21).
    All Cytokines Except For Human Il 2, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/all+cytokines+except+for+human+il-2/all+cytokines+except+for+human+il+2/pmc04156517-81-1-8
    Average 90 stars, based on 1 article reviews
    all cytokines except for human il-2 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy"

    Article Title: Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy

    Journal: Cancer immunology research

    doi: 10.1158/2326-6066.CIR-14-0038

    (a) Flow cytometric analysis of congenically marked OT-I CD8 T cells mixed ∼1:100 with bulk CD8 T cells and expanded with SIINFEKL-pulsed bone-marrow derived dendritic cells in IL-2 or cocktail of small molecules and cytokines (see methods). (b) Representative plots of expression of the phenotypic markers CD62L and CD44 on Thy1.2+ OT-I CD8 T cells. Plots in (a,b) representative of more than five independent experiments. (c) Plots of pre- and post-sort Thy1.2+ OT-I CD8 T cells grown as in (a) and purified by FACS for RNAseq analysis. (d) Unsupervised hierarchical clustering of RNAseq of populations sorted as in (c) showing all genes with a >2 fold difference between groups 1 and 3. Inset: principal component analysis of genes with >2-fold difference between any two groups for populations depicted in (c), and heatmap of selected biologically relevant genes in populations depicted in (c) and at selected time points of the OT-I response to L. monocytogenes Ova (from 21).
    Figure Legend Snippet: (a) Flow cytometric analysis of congenically marked OT-I CD8 T cells mixed ∼1:100 with bulk CD8 T cells and expanded with SIINFEKL-pulsed bone-marrow derived dendritic cells in IL-2 or cocktail of small molecules and cytokines (see methods). (b) Representative plots of expression of the phenotypic markers CD62L and CD44 on Thy1.2+ OT-I CD8 T cells. Plots in (a,b) representative of more than five independent experiments. (c) Plots of pre- and post-sort Thy1.2+ OT-I CD8 T cells grown as in (a) and purified by FACS for RNAseq analysis. (d) Unsupervised hierarchical clustering of RNAseq of populations sorted as in (c) showing all genes with a >2 fold difference between groups 1 and 3. Inset: principal component analysis of genes with >2-fold difference between any two groups for populations depicted in (c), and heatmap of selected biologically relevant genes in populations depicted in (c) and at selected time points of the OT-I response to L. monocytogenes Ova (from 21).

    Techniques Used: Derivative Assay, Expressing, Purification

    (a) Flow cytometric analysis of CD8 T cells cultured from CMV-seronegative HLA-A*0201+ PBMCs after pulldown with CMV-specific HLA-A*0201-pp65 dextramer. Cells were cultured in IL-2 or cocktail of memory-associated cytokines and small molecules. Plots depict dextramer-stained cells at pulldown and after 1, 2 or 3 weeks in culture with autologous antigen-pulsed monocyte-derived dendritic cells. (b) Representative plots of expression of the phenotypic markers of memory CCR7 and CD95 (Fas) on HLA-A*0201-pp65 dextramer positive gated cells. Plots in (a,b) representative of experiments performed with cells from two donors and two separate antigens. (c) Flow cytometric analysis of MACS-isolated CD8 T cells from human PBMCs expanded with CD3/CD28 antibody-coated beads in IL-2- or cocktail-containing media. Cells were labeled with Celltrace violet and divided cells were gated as shown for analysis of phenotypic markers CCR7 and CD95. Plots are representative of five independent experiments with PBMCs from three donors.
    Figure Legend Snippet: (a) Flow cytometric analysis of CD8 T cells cultured from CMV-seronegative HLA-A*0201+ PBMCs after pulldown with CMV-specific HLA-A*0201-pp65 dextramer. Cells were cultured in IL-2 or cocktail of memory-associated cytokines and small molecules. Plots depict dextramer-stained cells at pulldown and after 1, 2 or 3 weeks in culture with autologous antigen-pulsed monocyte-derived dendritic cells. (b) Representative plots of expression of the phenotypic markers of memory CCR7 and CD95 (Fas) on HLA-A*0201-pp65 dextramer positive gated cells. Plots in (a,b) representative of experiments performed with cells from two donors and two separate antigens. (c) Flow cytometric analysis of MACS-isolated CD8 T cells from human PBMCs expanded with CD3/CD28 antibody-coated beads in IL-2- or cocktail-containing media. Cells were labeled with Celltrace violet and divided cells were gated as shown for analysis of phenotypic markers CCR7 and CD95. Plots are representative of five independent experiments with PBMCs from three donors.

    Techniques Used: Cell Culture, Staining, Derivative Assay, Expressing, Isolation, Labeling

    Related Articles

    Derivative Assay:

    Article Title: Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy
    Article Snippet: All cytokines except for human IL-2 were from Peprotech.

    Expressing:

    Article Title: Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy
    Article Snippet: All cytokines except for human IL-2 were from Peprotech.

    Purification:

    Article Title: Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy
    Article Snippet: All cytokines except for human IL-2 were from Peprotech.

    Cell Culture:

    Article Title: Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy
    Article Snippet: All cytokines except for human IL-2 were from Peprotech.

    Staining:

    Article Title: Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy
    Article Snippet: All cytokines except for human IL-2 were from Peprotech.

    Isolation:

    Article Title: Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy
    Article Snippet: All cytokines except for human IL-2 were from Peprotech.

    Labeling:

    Article Title: Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy
    Article Snippet: All cytokines except for human IL-2 were from Peprotech.



    Similar Products

    all cytokines except for human il-2  (PeproTech)
    90
    PeproTech all cytokines except for human il-2
    (a) Flow cytometric analysis of congenically marked OT-I CD8 T cells mixed ∼1:100 with bulk CD8 T cells and expanded with SIINFEKL-pulsed bone-marrow derived dendritic cells in IL-2 or cocktail of small molecules and <t>cytokines</t> (see methods). (b) Representative plots of expression of the phenotypic markers CD62L and CD44 on Thy1.2+ OT-I CD8 T cells. Plots in (a,b) representative of more than five independent experiments. (c) Plots of pre- and post-sort Thy1.2+ OT-I CD8 T cells grown as in (a) and purified by FACS for RNAseq analysis. (d) Unsupervised hierarchical clustering of RNAseq of populations sorted as in (c) showing all genes with a >2 fold difference between groups 1 and 3. Inset: principal component analysis of genes with >2-fold difference between any two groups for populations depicted in (c), and heatmap of selected biologically relevant genes in populations depicted in (c) and at selected time points of the OT-I response to L. monocytogenes Ova (from 21).
    All Cytokines Except For Human Il 2, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/all+cytokines+except+for+human+il-2/all+cytokines+except+for+human+il+2/pmc04156517-81-1-8
    Average 90 stars, based on 1 article reviews
    all cytokines except for human il-2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) Flow cytometric analysis of congenically marked OT-I CD8 T cells mixed ∼1:100 with bulk CD8 T cells and expanded with SIINFEKL-pulsed bone-marrow derived dendritic cells in IL-2 or cocktail of small molecules and cytokines (see methods). (b) Representative plots of expression of the phenotypic markers CD62L and CD44 on Thy1.2+ OT-I CD8 T cells. Plots in (a,b) representative of more than five independent experiments. (c) Plots of pre- and post-sort Thy1.2+ OT-I CD8 T cells grown as in (a) and purified by FACS for RNAseq analysis. (d) Unsupervised hierarchical clustering of RNAseq of populations sorted as in (c) showing all genes with a >2 fold difference between groups 1 and 3. Inset: principal component analysis of genes with >2-fold difference between any two groups for populations depicted in (c), and heatmap of selected biologically relevant genes in populations depicted in (c) and at selected time points of the OT-I response to L. monocytogenes Ova (from 21).

    Journal: Cancer immunology research

    Article Title: Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy

    doi: 10.1158/2326-6066.CIR-14-0038

    Figure Lengend Snippet: (a) Flow cytometric analysis of congenically marked OT-I CD8 T cells mixed ∼1:100 with bulk CD8 T cells and expanded with SIINFEKL-pulsed bone-marrow derived dendritic cells in IL-2 or cocktail of small molecules and cytokines (see methods). (b) Representative plots of expression of the phenotypic markers CD62L and CD44 on Thy1.2+ OT-I CD8 T cells. Plots in (a,b) representative of more than five independent experiments. (c) Plots of pre- and post-sort Thy1.2+ OT-I CD8 T cells grown as in (a) and purified by FACS for RNAseq analysis. (d) Unsupervised hierarchical clustering of RNAseq of populations sorted as in (c) showing all genes with a >2 fold difference between groups 1 and 3. Inset: principal component analysis of genes with >2-fold difference between any two groups for populations depicted in (c), and heatmap of selected biologically relevant genes in populations depicted in (c) and at selected time points of the OT-I response to L. monocytogenes Ova (from 21).

    Article Snippet: All cytokines except for human IL-2 were from Peprotech.

    Techniques: Derivative Assay, Expressing, Purification

    (a) Flow cytometric analysis of CD8 T cells cultured from CMV-seronegative HLA-A*0201+ PBMCs after pulldown with CMV-specific HLA-A*0201-pp65 dextramer. Cells were cultured in IL-2 or cocktail of memory-associated cytokines and small molecules. Plots depict dextramer-stained cells at pulldown and after 1, 2 or 3 weeks in culture with autologous antigen-pulsed monocyte-derived dendritic cells. (b) Representative plots of expression of the phenotypic markers of memory CCR7 and CD95 (Fas) on HLA-A*0201-pp65 dextramer positive gated cells. Plots in (a,b) representative of experiments performed with cells from two donors and two separate antigens. (c) Flow cytometric analysis of MACS-isolated CD8 T cells from human PBMCs expanded with CD3/CD28 antibody-coated beads in IL-2- or cocktail-containing media. Cells were labeled with Celltrace violet and divided cells were gated as shown for analysis of phenotypic markers CCR7 and CD95. Plots are representative of five independent experiments with PBMCs from three donors.

    Journal: Cancer immunology research

    Article Title: Antigen-specific culture of memory-like CD8 T cells for adoptive immunotherapy

    doi: 10.1158/2326-6066.CIR-14-0038

    Figure Lengend Snippet: (a) Flow cytometric analysis of CD8 T cells cultured from CMV-seronegative HLA-A*0201+ PBMCs after pulldown with CMV-specific HLA-A*0201-pp65 dextramer. Cells were cultured in IL-2 or cocktail of memory-associated cytokines and small molecules. Plots depict dextramer-stained cells at pulldown and after 1, 2 or 3 weeks in culture with autologous antigen-pulsed monocyte-derived dendritic cells. (b) Representative plots of expression of the phenotypic markers of memory CCR7 and CD95 (Fas) on HLA-A*0201-pp65 dextramer positive gated cells. Plots in (a,b) representative of experiments performed with cells from two donors and two separate antigens. (c) Flow cytometric analysis of MACS-isolated CD8 T cells from human PBMCs expanded with CD3/CD28 antibody-coated beads in IL-2- or cocktail-containing media. Cells were labeled with Celltrace violet and divided cells were gated as shown for analysis of phenotypic markers CCR7 and CD95. Plots are representative of five independent experiments with PBMCs from three donors.

    Article Snippet: All cytokines except for human IL-2 were from Peprotech.

    Techniques: Cell Culture, Staining, Derivative Assay, Expressing, Isolation, Labeling